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Curcuma longa polyphenols improve insulin‐mediated lipid accumulation and attenuate proinflammatory response of 3T3‐L1 adipose cells during oxidative stress through regulation of key adipokines and antioxidant enzymes
Author(s) -
SeptembreMalaterre Axelle,
Le Sage Fanny,
Hatia Sarah,
Catan Aurélie,
Janci Laurent,
Gonthier MariePaule
Publication year - 2016
Publication title -
biofactors
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.204
H-Index - 94
eISSN - 1872-8081
pISSN - 0951-6433
DOI - 10.1002/biof.1288
Subject(s) - curcuma , oxidative stress , proinflammatory cytokine , curcumin , chemistry , antioxidant , superoxide dismutase , biochemistry , pharmacology , inflammation , biology , immunology , botany
Plant polyphenols may exert beneficial action against obesity‐related oxidative stress and inflammation which promote insulin resistance. This study evaluated the effect of polyphenols extracted from French Curcuma longa on 3T3‐L1 adipose cells exposed to H 2 O 2 ‐mediated oxidative stress. We found that Curcuma longa extract exhibited high amounts of curcuminoids identified as curcumin, demethoxycurcumin, and bisdemethoxycurcumin, which exerted free radical‐scavenging activities. Curcuma longa polyphenols improved insulin‐mediated lipid accumulation and upregulated peroxisome proliferator‐activated receptor‐gamma gene expression and adiponectin secretion which decreased in H 2 O 2 ‐treated cells. Curcuminoids attenuated H 2 O 2 ‐enhanced production of pro‐inflammatory molecules such as interleukin‐6, tumor necrosis factor‐alpha, monocyte chemoattractant protein‐1, and nuclear factor κappa B. Moreover, they reduced intracellular levels of reactive oxygen species elevated by H 2 O 2 and modulated the expression of genes encoding superoxide dismutase and catalase antioxidant enzymes. Collectively, these findings highlight that Curcuma longa polyphenols protect adipose cells against oxidative stress and may improve obesity‐related metabolic disorders. © 2016 BioFactors, 42(4):418–430, 2016