Premium
Neuroprotective effects of resveratrol on damages of mouse cortical neurons induced by β‐amyloid through activation of SIRT1/Akt1 pathway
Author(s) -
Zhang Jing,
Feng Xiaowen,
Wu Jintao,
Xu Hongyan,
Li Guibao,
Zhu Dexiao,
Yue Qingwei,
Liu Haili,
Zhang Yi,
Sun Dong,
Wang Hui,
Sun Jinhao
Publication year - 2013
Publication title -
biofactors
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.204
H-Index - 94
eISSN - 1872-8081
pISSN - 0951-6433
DOI - 10.1002/biof.1149
Subject(s) - resveratrol , neuroprotection , neurotoxicity , viability assay , phytoalexin , chemistry , pharmacology , reactive oxygen species , apoptosis , biochemistry , antioxidant , lactate dehydrogenase , microbiology and biotechnology , biology , toxicity , enzyme , organic chemistry
Resveratrol (3,5,4'‐tihydroxy‐trans‐stilbene), a polyphenolic phytoalexin found in the skin and seeds of grapes, has been reported to possess a wide range of biological and pharmacological activities including antioxidant, anti‐inflammatory, and antimutagenic effects. The present study intended to explore the neuroprotective effects of resveratrol against Aβ 25–35 ‐induced neurotoxicity of cultured mouse cortical neurons and the possible mechanisms involved. For this purpose, mouse cortical neurons were cultured and exposed to 30 μM Aβ 25–35 in the absence or presence of resveratrol (5, 10, and 25 μM). In addition, the potential contribution of the SIRT1/Akt1 neuroprotective pathway in resveratrol‐mediated protection against Aβ 25–35 ‐induced neurotoxicity was also investigated. The results showed that resveratrol dose‐dependently increased cell viability and reduced the number of apoptotic cells as measured by 3‐[4,5‐dimethylthiazol‐2‐yl]‐2, 5‐diphenyltetrazolium bromide (MTT) assay, lactate dehydrogenase (LDH) activity assay, reactive oxygen species (ROS) activity assay, and Hoechst/PI double staining. Further study revealed that resveratrol through activation of SIRT1/Akt1 to avert apoptosis. These findings raise the possibility that resveratrol may be a potent therapeutic compound against the neurodegenerative diseases. © 2013 BioFactors, 40(2):258–267, 2014