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Inhibitory effect of luteolin on the odorant‐induced cAMP level in HEK293 cells expressing the olfactory receptor
Author(s) -
Yoon Yeo Cho,
Hwang JinTeak,
Sung MiJeong,
Wang Shuaiyu,
Munkhtugs Davaatseren,
Rhyu MeeRa,
Park JaeHo
Publication year - 2012
Publication title -
biofactors
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.204
H-Index - 94
eISSN - 1872-8081
pISSN - 0951-6433
DOI - 10.1002/biof.1025
Subject(s) - luteolin , adenylyl cyclase , signal transduction , chemistry , olfactory system , cyclic adenosine monophosphate , receptor , biochemistry , microbiology and biotechnology , pharmacology , biology , flavonoid , neuroscience , antioxidant
Luteolin is a flavonoid in many fruits and vegetables. Although luteolin has important biological functions, including antioxidant, anti‐inflammatory, antimicrobial, and neuroprotective activities, little is known about the functions of luteolin in the olfactory system. Various odorants can be detected and distinguished by using several molecular processes, including the binding of odorants to odorant receptors, activation of adenylyl cyclase (AC), changes of cyclic adenosine monophosphate (cAMP) and Ca 2+ levels in olfactory sensory neurons, as well as changes in membrane potentials and the transmission of electric signals to the brain. Because AC‐cAMP signal transduction plays a pivotal role in the olfactory system, we evaluated the effects of luteolin on the AC‐cAMP pathway that had been stimulated by the odorant eugenol. We demonstrated that eugenol caused an upregulation of the cAMP level and the phosphorylation of phosphokinase A (PKA, a downstream target of cAMP) in human embryonic kidney 293 (HEK293) cells expressing the murine eugenol receptor. This upregulation significantly decreased in the presence of luteolin, suggesting that luteolin inhibited the odorant‐induced production of cAMP and affected the downstream phosphorylation of PKA.