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Ultrasensitive assay of clindamycin in medicine and bio‐fluids with chemiluminescence detection
Author(s) -
Wei Gaili,
Dang Gaochao,
Li Hua
Publication year - 2007
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.996
Subject(s) - chemiluminescence , chromatography , clindamycin , chemistry , antibiotics , biochemistry
A simple chemiluminescence (CL) method using flow injection has been developed for the determination of clindamycin, based on the inhibitory effect of clindamycin on the CL generated from the luminol–K 3 Fe(CN) 6 system in alkaline medium. It was found that the decrement of CL intensity was linear with the logarithm of clindamycin concentration over the range 0.7–1000 ng/mL. The detection limit was 0.2 ng/mL with a relative standard deviation (RSD) of <5.0% ( n = 7). At a flow rate of 3.0 mL/min, a complete analytical process could be performed within 0.5 min, including sampling and washing. The proposed procedure was applied successfully to the determination of clindamycin in pharmaceutical preparations and human urine without pretreatment. Copyright © 2007 John Wiley & Sons, Ltd.