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An attempt to design an isoluminol–hydrogen peroxidase‐amplified CL that measures intracellularly produced H 2 O 2 in phagocytes: sensitivity for H 2 O 2 is not high enough to allow detection
Author(s) -
Stenfeldt AnnaLena,
Dahlgren Claes
Publication year - 2007
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.990
Subject(s) - hydrogen peroxide , chemiluminescence , peroxidase , chemistry , sensitivity (control systems) , hydrogen , superoxide , photochemistry , analytical chemistry (journal) , enzyme , biochemistry , chromatography , organic chemistry , engineering , electronic engineering
A technique was designed for the determination of hydrogen peroxide release. We found, however, that the isoluminol‐amplified chemiluminescence technique is a suitable tool for measuring secretion of superoxide but not hydrogen peroxide. Copyright © 2007 John Wiley & Sons, Ltd.