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A flow‐injection chemiluminescence method for the determination of human serum albumin, using the reaction of fluorescein–human serum albumin–sodium hypochlorite by the enhancement effect of the cationic surfactant cetyltrimethylammonium bromide
Author(s) -
Huang ChunBao,
Zhang Kai,
Liu XueLian,
Wang ShengFu
Publication year - 2007
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.975
Subject(s) - chemistry , chemiluminescence , fluorescein , human serum albumin , pulmonary surfactant , chromatography , cationic polymerization , bromide , sodium hypochlorite , detection limit , rose bengal , fluorescence , biochemistry , inorganic chemistry , organic chemistry , physics , quantum mechanics
The interaction between surfactant and fluorescein was studied, using a fluorescence spectroscopy and flow‐injection (FI) chemiluminescence (CL) method. It was found that the cationic surfactant cetyltrimethylammonium bromide (CTAB) could cause the structural transformation of fluorescein from the quinone to the spirolactone form, and greatly enhance the CL intensity of the fluorescein–human serum albumin (HSA) complex. Based on this finding, a rapid and sensitive FI–CL method was developed for the determination of HSA. Under the optimum conditions, the proposed method has a linear range of 0.05–24.0 µg/mL, with a detection limit of 0.03 µg/mL for HSA (3 σ ). The relative standard deviation (RSD) of 1.2 µg/mL HSA ( n = 8) is 0.8%. The method was applied to the determination of protein content in urine samples, with satisfactory results. Density functional theory was used to study the mechanism of surfactant‐enhanced CL intensity of the fluorescein–HSA complex. Copyright © 2007 John Wiley & Sons, Ltd.