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Chemiluminescence assay for tetrahydrobiopterin based on the generation of hydrogen peroxide using isoluminol‐microperoxidase in the presence of 1‐methoxy PMS
Author(s) -
Arakawa Hidetoshi,
Masuda Kouichi,
Tajima Noriko,
Maeda Masako
Publication year - 2007
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.958
Subject(s) - chemiluminescence , hydrogen peroxide , luminol , chemistry , reactive oxygen species , oxygen , tetrahydrobiopterin , active oxygen , photochemistry , combinatorial chemistry , chromatography , organic chemistry , biochemistry , enzyme , cofactor
Abstract We developed a novel highly sensitive chemiluminescence (CL) method for BH 4 . The principle of the proposed method is based on active oxygen formation induced by 1‐methoxy‐5‐methyl phenazinium methyl sulphate (1‐methoxy PMS) in the presence of dissolved oxygen. Furthermore, active oxygen is determined by a CL assay involving the luminol reaction with microperoxidase. In this report, we examined the mechanism of formation and identified the reactive oxygen species derived from BH 4 employing 1‐methoxy PMS. Additionally, optimum conditions for the CL assay of BH 4 were established. Copyright © 2007 John Wiley & Sons, Ltd.