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Bioluminescent signal system: bioluminescence immunoassay of pathogenic organisms
Author(s) -
Frank L.,
Markova S.,
Remmel N.,
Vysotski E.,
Gitelson I.
Publication year - 2007
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.952
Subject(s) - immunoassay , bioluminescence , chromatography , photoprotein , chemistry , shigella , escherichia coli , hbsag , microbiology and biotechnology , detection limit , antibody , virology , biology , hepatitis b virus , biochemistry , virus , immunology , gene
The Ca 2+ ‐regulated photoprotein obelin has been examined as a label for bioluminescence immunoassay of infective agents. The hepatitis B virus (HbsAg) and the bacteria Escherichia coli and Shigella sonnei lipopolysaccharide (LPS) were chosen as model antigens. Chemically synthesized obelin‐corresponding antibody conjugates were used in a solid‐phase microplate immunoassay. The sensitivities achieved by the assay were 0.25 ng/mL for S. sonnei LPS and 0.375 ng/mL for HbsAg. A novel, filter‐based immunoassay to determine bacterial admixtures in the environment was proposed. The NanoCeram filters were effectively applied to ‘trap’ and pre‐concentrate pathogens from samples under study for the purposes of further detection and measurement of the absorbed material by bioluminescence immunoassay. Copyright © 2007 John Wiley & Sons, Ltd.