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Protein analysis with terbium (III) and sodium dodecyl sulphonate by a second‐order scattering technique
Author(s) -
Li Aiyun,
Zhao Huichun,
Jin Linpei
Publication year - 2006
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.920
Subject(s) - terbium , chemistry , coomassie brilliant blue , chromatography , sodium , human serum albumin , detection limit , blood proteins , sodium dodecyl sulfate , analytical chemistry (journal) , nuclear chemistry , biochemistry , staining , ion , biology , organic chemistry , genetics
This is the first report of terbium(III) as a probe of second‐order scattering (SOS) for the determination of proteins in human serum at nanogram levels. A sensitive method has been developed using light scattering, based on the fact that the weak SOS of proteins can be enhanced in the presence of terbium(III) and sodium dodecyl sulphonate (SDS). With this method, 7.0 × 10 −9 –1.0 × 10 −5 g/mL human serum albumin (HSA) and 5.0 × 10 −9 –5.0 × 10 −6 g/mL γ ‐globulin can be determined; the detection limits were 4.4 ng/mL for HSA and 3.1 ng/mL for γ ‐globulin. The method has been applied to the detection of total proteins in human serum samples, and the results are consistent with those obtained by the Coomassie brilliant blue (CBB) G‐250 assay. Copyright © 2006 John Wiley & Sons, Ltd.