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Inhibitory effect of stobadine on FMLP‐induced chemiluminescence in human whole blood and isolated polymorphonuclear leukocytes
Author(s) -
Drábiková Katarína,
Jančinová Viera,
Nosál Radomír,
Pečivová Jana,
Mačičková Tatiana,
Turčáni Peter
Publication year - 2006
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.919
Subject(s) - chemiluminescence , chemistry , inhibitory postsynaptic potential , whole blood , granulocyte , neutrophile , luminol , immunology , medicine , biochemistry , chromatography , in vitro
The chemiluminescence (CL) technique with luminol and isoluminol was used to characterize the effect of stobadine on reactive oxygen metabolites (ROM) generation in human whole blood and in isolated polymorphonuclear leukocytes (PMNL) stimulated with N‐formyl‐methionyl‐leucyl‐phenyl‐alanine (FMLP). In whole blood and in isolated PMNL, stobadine in the concentrations of 1, 10 and 100 µmol/L significantly inhibited the CL signal after FMLP, which activated predominantly extracellular generation of ROM. The same concentrations of stobadine were effective on CL in a cell‐free system. On the other hand, myeloperoxidase (MPO) liberation was decreased by stobadine only in the concentration of 100 µmol/L. The results showed stobadine to act as a potent inhibitor/scavenger of extracellularly produced ROM in human PMNL and indicated interference of stobadine with ROM as well as with signalling events resulting in NADPH‐oxidase activation and MPO liberation. Copyright © 2006 John Wiley & Sons, Ltd.