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A comparison of chemical systems for luminometric determination of antioxidant capacity towards individual reactive oxygen species
Author(s) -
Komrskova Daniela,
Lojek Antonin,
Hrbac Jan,
Ciz Milan
Publication year - 2006
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.913
Subject(s) - chemistry , hydrogen peroxide , reactive oxygen species , chemiluminescence , luminol , oxygen , superoxide dismutase , catalase , nuclear chemistry , electron paramagnetic resonance , inorganic chemistry , iodometry , antioxidant , biochemistry , chromatography , nuclear magnetic resonance , organic chemistry , physics
The aim of the study was to investigate the reactive oxygen species (ROS) production in the hypoxanthine–xanthinoxidase (HX–XO), hydrogen peroxide–ferrous sulphate (H 2 O 2 –FeSO 4 ) and hydrogen peroxide (H 2 O 2 ) systems by using various concentrations of ROS scavengers, such as superoxide dismutase (SOD), dimethylthiourea (DMTU) or catalase (CAT). Luminol (0.8 mmol/L) was dissolved in a borate buffer, pH 9.0, and was used as a luminophor in the chemiluminescence (CL) measurements. In the HX–XO system SOD, CAT and DMTU deepened the CL signal, whereas in the H 2 O 2 –FeSO 4 system, only CAT and DMTU deepened the CL signal, and in the H 2 O 2 system SOD and CAT increased and DMTU deepened the CL signal. Electron spin resonance (ESR) measurements were performed only in the H 2 O 2 –FeSO 4 system. 5,5‐dimethyl‐pyrroline‐N‐oxide (DMPO) was used as a spin trap. According to typical ESR spectra, .OH was produced in this chemical system. It can be concluded that the chemical systems do not produce single reactive oxygen species but a mixture of them. Copyright © 2006 John Wiley & Sons, Ltd.