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The sensitive determination of nucleic acids using a fluorescence‐quenching method
Author(s) -
Jia Zhen,
Yang Jinghe,
Wu Xia,
Sun Changxia,
Wang Fei,
Liu Shufang,
Wang Feng
Publication year - 2006
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.906
Subject(s) - nucleic acid , thenoyltrifluoroacetone , chemistry , fluorescence , quenching (fluorescence) , nucleic acid quantitation , dna , chromatography , rna , detection limit , fish <actinopterygii> , biochemistry , biology , extraction (chemistry) , gene , fishery , physics , quantum mechanics , solvent extraction
Experiments indicated that nucleic acids can quench the fluorescence of the Eu 3+ –2‐thenoyltrifluoroacetone (TTA)–1,10‐phenanthroline (Phen) system. Based on this, a sensitive method for the determination of nucleic acids was proposed. The experiments indicated that under the optimum conditions, the quenched fluorescence intensity was in proportion to the concentration of nucleic acids in the range 1.0 × 10 −11 –1.0 × 10 −6 g/mL for yeast RNA (yRNA), 5.0 × 10 −11 –5.0 × 10 −7 g/mL for fish sperm (fsDNA) and 1.0 × 10 −10 –1.5 × 10 −6 g/mL for calf thymus DNA (ctDNA). Their detection limits were 3.0 × 10 −12 , 4.0 × 10 −12 and 5.0 × 10 −11 g/mL, respectively. Therefore, the proposed method is one of the most sensitive methods available. The interaction between nucleic acids and Eu 3+ ‐TTA‐Phen is also discussed. Copyright © 2006 John Wiley & Sons, Ltd.