Determination of nucleic acids based on the quenching effect on resonance light scattering of the Y(III)–1,6‐bi(1′‐phenyl‐3′‐methyl‐5′‐pyrazolone‐4′‐)hexane‐dione system

Nucleic acids can quench resonance light scattering (RLS) intensity of the Y(III)–1,6‐bi(1′‐phenyl‐3′‐methyl‐5′‐pyrazolone‐4′‐)hexane‐dione(BPMPHD) complex in the pH range 5.0–5.8. Under optimal conditions, there are linear relationships between the quenching of RLS and the concentration of nucleic acids in the range 6.3 × 10 −8 –2.1 × 10 −5 g/mL for fish sperm DNA (fsDNA), 1.2 × 10 −8 –5.0 × 10 −5 g/mL for calf thymus DNA (ctDNA) and 6.0 × 10 −8 –2.0 × 10 −5 g/mL for yeast RNA (yRNA). The detection limits (3 s) of fsDNA, ctDNA and yRNA are 0.7 ng/mL, 3.8 ng/mL and 4.2 ng/mL, respectively. Copyright © 2006 John Wiley & Sons, Ltd.