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Fluorescence enhancement effect of the morin–Al 3+ –sodium dodecyl benzene sulphonate–protein system and the determination of proteins
Author(s) -
Wang Fei,
Yang Jinghe,
Wu Xia,
Sun Changxia,
Liu Shufang,
Wang Feng,
Jia Zhen
Publication year - 2006
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.883
Subject(s) - chemistry , morin , fluorescence , bovine serum albumin , sodium , human serum albumin , benzene , chromatography , sodium dodecyl sulfate , egg albumin , serum albumin , biochemistry , organic chemistry , medicine , physics , pathology , quantum mechanics
Abstract The fluorescence intensity of the morin–Al 3+ complex was greatly enhanced by proteins in the presence of sodium dodecyl benzene sulphonate (SDBS). Based on this, a new fluorimetric method for the determination of protein was developed. Under optimum conditions, the enhanced intensity of fluorescence was in proportion to the concentration of proteins in the range 1.0 × 10 −8 –1.3 × 10 −5 g/mL for bovine serum albumin (BSA), 4.0 × 10 −8 –1.2 × 10 −5 g/mL for egg albumin (EA) and 5.0 × 10 −8 –1.2 × 10 −5 g/mL for human serum albumin (HSA). Their detection limits (S:N = 3) were 5.0 × 10 −9 , 1.8 × 10 −8 and 1.6 × 10 −8 g/mL, respectively. The interaction mechanism was also studied. Copyright © 2005 John Wiley & Sons, Ltd.