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Simultaneous measurement of superoxide generation and intracellular calcium ion of neutrophil‐like culture cells
Author(s) -
Satozono Hiroshi,
Kazumura Kimiko,
Okazaki Sigetoshi,
Hiramatsu Mitsuo
Publication year - 2005
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.882
Subject(s) - superoxide , calcium , intracellular , chemistry , calcium in biology , microbiology and biotechnology , biochemistry , biology , organic chemistry , enzyme
We have developed a novel method for simultaneously measuring fluorescence and chemiluminescence. The generation of superoxide anion and the intracellular Ca 2+ ion concentration of neutrophil‐like cells stimulated by agonists were measured in real time by our method. Our results were in agreement with the intracellular signalling in the neutrophils. We also found that the presence of Zn 2+ ion inhibited both the generation of superoxide anion and the influx of Ca 2+ ions. Copyright © 2005 John Wiley & Sons, Ltd.