Premium
Simple electrospray mass spectrometry detection of acylhomoserine lactones
Author(s) -
Makemson John,
Eberhard Anatol,
Mathee Kalai
Publication year - 2005
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.873
Subject(s) - chemistry , mass spectrometry , electrospray , electrospray mass spectrometry , tandem mass spectrometry , protonation , reactivity (psychology) , molecule , ammonium acetate , chromatography , ion , organic chemistry , medicine , alternative medicine , pathology , high performance liquid chromatography
Simple (non‐tandem) electrospray mass spectrometry (ESMS) can detect acyl homoserine lactones (AHL) in bacteriological media in picomole amounts. The chemical reactivity of AHLs and their extraction behaviour into ethyl acetate, coupled with detection in the ESMS, has shown that these lactones can be detected as the protonated pseudomolecular ions themselves as well as solvent and ammonium adducts, and as dimers. ESMS detects and identifies these molecules, utilizing simple chemical properties of AHLs. Copyright © 2005 John Wiley & Sons, Ltd.