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Comparative study of antioxidants as quenchers or scavengers of reactive oxygen species based on quenching of MCLA‐dependent chemiluminescence
Author(s) -
Hosaka S.,
Obuki M.,
Nakajima J.,
Suzuki M.
Publication year - 2005
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.867
Subject(s) - ascorbic acid , chemistry , chemiluminescence , reactive oxygen species , quenching (fluorescence) , antioxidant , scavenger , beta carotene , lycopene , astaxanthin , tocopherol , radical , biochemistry , free radical scavenger , carotenoid , photochemistry , vitamin e , chromatography , food science , fluorescence , physics , quantum mechanics
The quenching or scavenging effect of non‐enzymatic antioxidants against reactive oxygen species (ROS) was studied by comparing the degree of suppression of chemiluminescence (CL) caused by the oxidation of MCLA (methoxylated Cypridina luciferin analogue) by ROS. MCLA‐dependent CL caused by O 2 − was effectively quenched by ascorbic acid, β ‐carotene, lycopene and astaxanthin, while it was enhanced by α ‐tocopherol. The CL by 1 O 2 was quenched effectively by β ‐carotene, lycopene and astaxanthin, moderately by ascorbic acid, and slightly by α ‐tocopherol. β ‐Carotene and α ‐tocopherol remarkably suppressed the CL when ROS was HO • . The present study revealed that MCLA‐dependent CL assay provides a simple and rapid method for the evaluation of antioxidants as a quencher or scavenger against any kind of ROS. Copyright © 2005 John Wiley & Sons, Ltd.