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Chemiluminescence determination of pharmacologically active compounds by capillary electrophoresis
Author(s) -
Han Suqin
Publication year - 2005
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.863
Subject(s) - chromatography , chemistry , luminol , capillary electrophoresis , chemiluminescence , detection limit , protocatechuic acid , rutin , analyte , chlorogenic acid , organic chemistry , antioxidant
A simple and rapid capillary electrophoresis with direct chemiluminescence method has been developed for the determination of five natural pharmacologically active compounds including rutin, protocatechuic aldehyde, chlorogenic acid, luteolin and protocatechuic acid. The luminol as a component of the separation electrolyte buffer was introduced at the head of the separation capillary. The separation of five compounds was carried out in a fused‐silica capillary with 15.0 mmol/L tetraborate, 1.0 mmol/L SDS and 0.42 mmol/L luminol (pH 8.5). The analytes was determined by enhancing the chemiluminescence of luminol with 0.13 mmol/L K 3 Fe(CN) 6 in 0.05 mol/L NaOH, which was introduced at the post‐column stage. The voltage applied was 16 kV. Under the optimum conditions, the analytes were separated within 10 min. The excellent linearity was obtained over two to three orders of magnitude with a detection limit (signal:noise = 3) of 0.012–0.055 µmol/L for all five analytes. The method was successfully used in the analysis of pharmaceutical and biological samples, and the assay results were satisfactory. Copyright © 2005 John Wiley & Sons, Ltd.