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A novel chemiluminescence method for the determination of orciprenaline based on ferricyanide‐rhodamine 6G
Author(s) -
Lv Yi,
Zhao Rui,
Zhu Zhenli,
Xu Xianshun,
Zhang Xinrong
Publication year - 2005
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.860
Subject(s) - orciprenaline , chemiluminescence , potassium ferricyanide , chemistry , detection limit , ferricyanide , relative standard deviation , rhodamine 6g , chromatography , flow injection analysis , analytical chemistry (journal) , inorganic chemistry , organic chemistry , anesthesia , medicine , molecule
A novel flow injection chemiluminescence method for the determination of orciprenaline was developed. The method is based on the chemiluminescence (CL) reaction of orciprenaline with potassium ferricyanide in sodium hydroxide medium, sensitized by the fluorescent dye rhodamine 6G. The proposed procedure allows quantitation of orciprenaline in the concentration range 0.01–1.2 µg/mL, with a detection limit of 7.2 × 10 −3 µg/mL. The relative standard deviation (RSD) is 2.7% for 0.1 µg/mL orciprenaline ( n = 9). The sampling frequency was calculated at ∼120/h. The method was successfully applied to the determination of orciprenaline in pharmaceutical preparations. A brief discussion on the possible CL reaction mechanism is presented. Copyright © 2005 John Wiley & Sons, Ltd.