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Photochemiluminescent detection of antiradical activity. VII. Comparison with a modified method of thermo‐initiated free radical generation with chemiluminescent detection
Author(s) -
Popov I.,
Lewin G.
Publication year - 2005
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.856
Subject(s) - chemiluminescence , chemistry , luminol , radical , chromatography , luminescent measurements , superoxide dismutase , lipid oxidation , antioxidant , biochemistry , materials science , luminescence , optoelectronics
The method of photosensitized chemiluminescence (PCL) allows the quantification of water‐ and lipid‐soluble antioxidants and activity of superoxide dismutase (SOD) in the same measuring system. However, it needs a special device, which we have described in a previous paper in this series. Another method suitable for the assay of water‐ and lipid‐soluble antioxidants is the thermo‐initiated decay of azo‐compounds combined with the measurement of O 2 consumption (Niki, 1985; Wayner et al. , 1985). Its long duration and the complicated measuring procedure is not acceptable for routine medical applications. We show that a modification using CL detection of free radicals with luminol, has results comparable with PCL for the determination of non‐enzymic water‐ and lipid‐soluble antioxidants, SOD activity and oxidative modification of proteins. In contrast to PCL, it is possible to use any luminometer with a heatable measuring cell and to investigate coloured samples. While the new method has an overall higher sensitivity and is scalable to microtitre plates, PCL measurements can be made at different pH. The advantages and analytical information content of certain components of the integral antioxidative capacity of blood plasma are discussed in comparison with other methods. Copyright © 2005 John Wiley & Sons, Ltd.