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Flow‐injection with enhanced chemiluminescence detection of ofloxacin in human plasma
Author(s) -
Zhang Xianhua,
Ouyang Jin,
Zhai Suodi,
Huang Guangming,
Zhao Huichun
Publication year - 2005
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.853
Subject(s) - ofloxacin , chemiluminescence , detection limit , chromatography , chemistry , pharmacokinetics , flow injection analysis , acetonitrile , plasma , pharmacology , antibiotics , medicine , biochemistry , ciprofloxacin , physics , quantum mechanics
A simple, rapid and sensitive method for the determination of ofloxacin in plasma has been developed based on flow‐injection analysis with enhanced chemiluminescence (CL) detection. This method employs the CL reaction of cerium(IV) and sulphite sensitized by a Tb(III)–ofloxacin complex. Plasma samples were deproteinized with acetonitrile before analysis. Under optimal conditions, the method allowed the quantitative analysis of ofloxacin in plasma over the range of 22.5–900 ng/mL with a detection limit of 7.5 ng/mL (3 σ ). The intra‐day and inter‐day precision was < 3.0% ( n = 11) and < 7.0% ( n = 3), respectively. The method was a convenient tool, rapid (20 s/analysis) and well suited for pharmacokinetics studies in which thousands of samples must be analysed daily. It was applied to pharmacokinetics studies of ofloxacin. Copyright © 2005 John Wiley & Sons, Ltd.