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Chemiluminescence microflow injection analysis system on a chip for the determination of uric acid without enzyme
Author(s) -
He Deyong,
Zhang Zhujun,
Huang Ying,
Hu Yufei,
Zhou Houjiang,
Chen Dinglong
Publication year - 2005
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.847
Subject(s) - chemiluminescence , uric acid , chemistry , chromatography , enzyme , biochemistry
A new microflow injection analysis ( µ FIA) system on a chip coupled with chemiluminescence (CL) for the non‐enzymatic determination of uric acid is described. The µ FIA system produced by using two transparent poly(methylmethacrylate) (PMMA) chips measured 50 × 40 × 5 mm, the microchannels, etched by CO 2 laser, were 200 µm wide and 100 µm deep, and the volume of the reaction area (RA) was about 1.2 µL. The injection pump, with accurate time control, monitored all reagents, including the sample. The uric acid was sensed by the chemiluminescence reaction between luminol and ferricyanide. The linear range of the uric acid concentration was 0.8–30 mg/L and the detection limit was 0.5 mg/L (S/N = 3). The relative standard deviation was 4.42% for 5 mg/L uric acid ( n = 8). The proposed method has been successfully applied to the non‐separation determination of uric acid in human serum and urine. Copyright © 2005 John Wiley & Sons, Ltd.