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Spectral and kinetic studies on the interaction between oxyhaemoglobin and peroxynitrite: a comparison with hydrogen peroxide
Author(s) -
Wang Ruiyong,
Ji Mina,
Cai Ruxiu,
Lu Yingtang
Publication year - 2005
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.842
Subject(s) - peroxynitrite , chemistry , absorbance , hydrogen peroxide , kinetic energy , spectrophotometry , photochemistry , kinetics , reaction rate constant , decomposition , inorganic chemistry , analytical chemistry (journal) , chromatography , organic chemistry , physics , quantum mechanics , enzyme , superoxide
Interaction between oxyhaemoglobin and peroxynitrite was studied using stopped‐flow rapid‐scan spectrophotometry. The influence of pH, peroxynitrite concentration and temperature on the pseudo‐first‐order rate constants was studied and the activation energy calculated. The kinetic curve for the oxyhaemoglobin–peroxynitrite reaction showed that a fast reaction occurred in the initial seconds, followed by a slow process of decrease in absorbance. The biphasic reaction kinetics of oxyhaemoglobin with peroxynitrite or hydrogen peroxide demonstrated the existence of an intermediary species. For the first time a rapid‐scan stopped‐flow spectrophotometry study is presented, yielding spectral and kinetic data of the reaction. Copyright © 2005 John Wiley & Sons, Ltd.