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The fluorescence sensor for saccharide based on internal conversion
Author(s) -
Sun XiangYing,
Liu Bin
Publication year - 2005
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.841
Subject(s) - chemistry , fluorescence , fructose , quantum yield , boronic acid , yield (engineering) , solvent , buffer solution , photochemistry , chromatography , organic chemistry , materials science , physics , quantum mechanics , metallurgy
In this paper, an internal conversion (IC) fluorescence probe N‐( o ‐boronic acid)benzyl‐1‐naphthylamine (BBNA) was prepared from 1‐naphthylamine and 2‐formylbenzeneboronic acid. The fluorescence parameters of BBNA were investigated in a variety of solvents. When BBNA interacted with d ‐fructose in phosphate buffer solution of 30% MeOH, pH 8.21 (v/v), the fluorescence intensity increased and emission maximum red‐shifted slightly with increasing d ‐fructose concentration. In the presence of d ‐fructose, the fluorescence quantum yield of BBNA increased with increasing solvent polarity, suggesting that internal conversion (IC) occurred with BBNA. The binding force of BBNA with d ‐fructose was the strongest, and the stability constant ( K ) of d ‐fructose was 99.9 mol/L. Therefore, a selective recognition system based on IC was constructed for d ‐fructose. Copyright © 2005 John Wiley & Sons, Ltd.