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Flow‐injection–chemiluminescence method for the determination of penicillin G potassium
Author(s) -
Cao Wei,
Yang JingHe,
Sun ChangXia,
Zhang ZhiJuang,
Gao QingFei
Publication year - 2005
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.839
Subject(s) - chemiluminescence , potassium permanganate , chemistry , potassium , penicillin , detection limit , chromatography , flow injection analysis , inorganic chemistry , biochemistry , organic chemistry , antibiotics
The degradation product of penicillin G potassium can react with potassium permanganate in acidic medium and produce chemiluminescence, which is greatly enhanced by formaldehyde. The optimum conditions for this chemiluminescent reaction were studied in detail using a flow‐injection system. The experiments indicated that under optimum conditions, the chemiluminescence intensity was linearly related to the concentration of penicillin G potassium within the range 1.0 × 10 −7 –1.0 × 10 −5 g/mL, with a detection limit (3 σ ) of 7 × 10 −8 g/mL. The relative standard deviation was 1.0% for 4.0 × 10 −7 g/mL penicillin G potassium solution ( n = 11). This method has the advantages of simple operation, fast response and high sensitivity. The method was successfully applied to the analysis of penicillin G potassium in raw medicines. Copyright © 2005 John Wiley & Sons, Ltd.