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Flow‐injection resonance light scattering detection of proteins at the nanogram level
Author(s) -
Tan Kejun,
Li Yuanfang,
Huang Chengzhi
Publication year - 2005
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.831
Subject(s) - bovine serum albumin , human serum albumin , chemistry , chromatography , detection limit , light scattering , resonance (particle physics) , serum albumin , aqueous solution , albumin , analytical chemistry (journal) , scattering , biochemistry , optics , physics , particle physics
A resonance light scattering (RLS) detection method for protein was developed, using a flow‐injection system based on the enhancement of RLS signals from Biebrich scarlet (BS) by protein. The enhanced RLS intensities at 286.0 nm, in acidic aqueous medium, were proportional to the protein concentration over the range 0.005–18 µg[sol ]mL and 0.008–16 µg[sol ]mL for human serum albumin (HSA) and bovine serum albumin (BSA), respectively, with corresponding limits of detection (3 σ ) of 5.00 ng[sol ]mL for HSA, and 7.80 ng[sol ]mL for BSA. The method was successfully applied to the quantification of total proteins in human serum samples. Copyright © 2005 John Wiley & Sons, Ltd.