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Determination of oxytetracycline by flow injection with chemiluminescence detection
Author(s) -
Guo Liangqia,
Xie Zenghong,
Bian Xintong,
Lin Xucong,
Zhang Weilin,
Liu Xiaohua,
Chen and Guonan
Publication year - 2005
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.827
Subject(s) - chemiluminescence , oxytetracycline , ruthenium , cerium , detection limit , chemistry , flow injection analysis , tris , calibration curve , chromatography , linear range , nuclear chemistry , inorganic chemistry , catalysis , organic chemistry , biochemistry , antibiotics
A flow‐injection chemiluminescent method for the determination of oxytetracycline was developed. The method is based on an enhancement by oxytetracycline of the chemiluminescence light emission of tris(2,2′‐bipyridine) ruthenium (II), generated by the continuous oxidation of tris(2,2′‐bipyridine) ruthenium (II) by cerium (IV) sulphate in sulphuric acid. Under the optimum conditions, the calibration curve was linear over the range 1.0 × 10 −7 –1.0 × 10 −5 g[sol ]mL for oxytetracycline with the linear equation: Δ INT = 148.77 × C + 0.6637 ( R 2 = 0.9994). The detection limit was 4.52 × 10 −8 g[sol ]mL. The proposed method was also successfully used to determine oxytetracycline in pharmaceutical formulations. The mean recovery of determination of oxytetracycline was 92.73%. A mechanism for the chemiluminescence enhancement by oxytetracycline of tris(2,2′‐bipyridine)–ruthenium (II) and cerium (IV) sulphate system is also proposed. Copyright © 2005 John Wiley & Sons, Ltd.