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Chemiluminescence determination of fluoroquinolone antibiotics using a soluble manganese (IV)‐sulphite system
Author(s) -
Du Jianxiu,
Li Yinhuan,
Lu Jiuru
Publication year - 2005
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.798
Subject(s) - norfloxacin , ofloxacin , chemiluminescence , chemistry , ciprofloxacin , manganese , nuclear chemistry , chromatography , antibacterial agent , detection limit , antibiotics , organic chemistry , biochemistry
Abstract The reaction of soluble manganese (IV) with sulphite in acidic condition was found to elicit weak chemiluminescence (CL). The CL signal was remarkably enhanced in the presence of three fluoroquinolones, viz. norfloxacin, ofloxacin and ciprofloxacin. Based on these observations, a new flow‐injection CL method was developed for the determination of these fluoroquinolones. The method allows determination in the range 5.0 × 10 −8 –1.0 × 10 −6 mol[sol ]L for norfloxacin, 1.0 × 10 −7 –8.0 × 10 −6 mol[sol ]L for ofloxacin and 1.0 × 10 −7 –3.0 × 10 −5 mol[sol ]L for ciprofloxacin, with detection limits of 3 × 10 −8 mol[sol ]L, 5 × 10 −8 mol[sol ]L and 3 × 10 −8 mol[sol ]L, respectively. The method was applied to the determination of fluoroquinolones in pharmaceutical preparations. Copyright © 2005 John Wiley & Sons, Ltd.