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Effects of isoflurane on measurements of delayed lumininescence in Acetabularia acetabulum
Author(s) -
Chen Wen Li,
Wijk Roeland Van,
Xing Da
Publication year - 2005
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.794
Subject(s) - acetabulum , isoflurane , geology , environmental science , medicine , anesthesia , surgery
The volatile halogenated methyl ethyl ether, isoflurane, used as an anaesthetic, inhibits actin‐based dynamics directly or indirectly in animal cells. In plant cells, most intracellular movements are related to actin pathways. We have used isoflurane in a unicellular alga, Acetabularia acetabulum , to test the dynamics of choloroplast organization. By measuring the delayed luminescence, we found that isoflurane worked efficiently in the unicellular organism and showed dose‐ and time‐course‐dependent actin‐inhibition patterns. When A. acetabulum was treated with saturated solutions of isoflurane in artificial seawater (defined as 100% isoflurane) for 3 or 6 min, the delayed luminescence (DL) was decreased and was never recovered. In contrast, if treated with 75% diluted isoflurane, the DL was firstly inhibited and then recovered several hours later, and if treated with 50% diluted isoflurane, the change of DL was small. Our work proved that isoflurane can affect actin‐related pathways in both animals and plants. Copyright © 2005 John Wiley & Sons, Ltd.