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Study on the interaction between protein and Eu(III)–chlorotetracycline complex and the determination of protein using the fluorimetric method
Author(s) -
Liu Shufang,
Yang Jinghe,
Wu Xia,
Wang Fei,
Wang Feng,
Jia Zhen,
Mao Lijuan
Publication year - 2004
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.792
Subject(s) - chemistry , protein–protein interaction , chromatography , biochemistry
Chlorotetracycline (CTC) can react with europium ions Eu 3+ , and the complex emits the intrinsic uorescence of Eu 3+ . The intensity is greatly enhanced by proteins and this forms the basis of a new uorimetric method for determination of protein. Further research indicates that under optimum conditions, the enhanced intensity of uorescence is in proportion to the concentration of proteins, in the range 2.0 × 10 −7 –1.0 × 10 −5 g/mL for bovine serum albumin (BSA) (linear equation, I f = 34.35933 + 11.54467 × 10 6 C)( r = 0.99895) and 8.0 × 10 −7 –1.0 × 10 −5 g/mL for human serum albumin (HSA) (linear equation, I f = 76.58881 + 5.3569 × 10 6 C) ( r = 0.99283). Detection limits (S/N = 3) were 8.9 × 10 −9 g/mL for BSA and 3.3 × 10 −8 g/mL for HSA. In an assay for BSA in calf serum, this method gave a value close to that determined by the UV spectrophotometric method. Copyright © 2004 John Wiley & Sons, Ltd.