Chemiluminescent analysis of the antioxidant and immunomodulation effects of several psychotropic drugs on peritoneal macrophages | Zendy

Hadjimitova V. | Zendy; Traykov T. | Zendy; Bakalova R. | Zendy; Petrova V. | Zendy; Lambev I. | Zendy; Ohba H. | Zendy; Ishikawa M. | Zendy; Baba Y. | Zendy

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Chemiluminescent analysis of the antioxidant and immunomodulation effects of several psychotropic drugs on peritoneal macrophages

Author(s) -

Hadjimitova V.,

Traykov T.,

Bakalova R.,

Petrova V.,

Lambev I.,

Ohba H.,

Ishikawa M.,

Baba Y.

Publication year - 2004

Publication title -

luminescence

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.428

H-Index - 45

eISSN - 1522-7243

pISSN - 1522-7235

DOI - 10.1002/bio.788

Subject(s) - antioxidant , chemistry , chemiluminescence , superoxide , pharmacology , radical , drug , biochemistry , reactive oxygen species , medicine , enzyme , chromatography

The present study describes the application of several chemiluminescent (CL) methods for evaluation of antioxidant and immunomodulation effects of psychotropic drugs upon phagocytes: KO 2 ‐induced luminal‐dependent CL for detection of superoxide anion radicals in a pure chemical system; PMA‐ and A23187‐induced CL of peritoneal macrophages for detection of free radicals in cell suspension; and CL, produced by the luciferase‐catalyzed luciferin + ATP reaction, for evaluation of cell viability before and after drug application. These methods provide also a way to investigate the location of drug action. It was found that the psychotropic drugs inuence the ‘oxidative burst’ of macrophages through two mechanisms: by expression of drug antioxidant properties and/or by a direct immunomodulation effect. Copyright © 2004 John Wiley & Sons, Ltd.

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