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Chemiluminescent analysis of the antioxidant and immunomodulation effects of several psychotropic drugs on peritoneal macrophages
Author(s) -
Hadjimitova V.,
Traykov T.,
Bakalova R.,
Petrova V.,
Lambev I.,
Ohba H.,
Ishikawa M.,
Baba Y.
Publication year - 2004
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.788
Subject(s) - antioxidant , chemistry , chemiluminescence , superoxide , pharmacology , radical , drug , biochemistry , reactive oxygen species , medicine , enzyme , chromatography
Abstract The present study describes the application of several chemiluminescent (CL) methods for evaluation of antioxidant and immunomodulation effects of psychotropic drugs upon phagocytes: KO 2 ‐induced luminal‐dependent CL for detection of superoxide anion radicals in a pure chemical system; PMA‐ and A23187‐induced CL of peritoneal macrophages for detection of free radicals in cell suspension; and CL, produced by the luciferase‐catalyzed luciferin + ATP reaction, for evaluation of cell viability before and after drug application. These methods provide also a way to investigate the location of drug action. It was found that the psychotropic drugs inuence the ‘oxidative burst’ of macrophages through two mechanisms: by expression of drug antioxidant properties and/or by a direct immunomodulation effect. Copyright © 2004 John Wiley & Sons, Ltd.