Premium
Evaluation of the degree of medical radiation damage with a highly sensitive chemiluminescence method
Author(s) -
Zhu Debin,
Xing Da,
Wei Yadong,
Li Xian,
Gao Bo
Publication year - 2004
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.782
Subject(s) - chemiluminescence , superoxide dismutase , reactive oxygen species , sodium azide , irradiation , superoxide , catalase , ionizing radiation , chemistry , hela , singlet oxygen , biophysics , lymphocyte , oxygen , microbiology and biotechnology , biochemistry , radiochemistry , oxidative stress , cell , immunology , biology , enzyme , chromatography , physics , organic chemistry , nuclear physics
Abstract A highly sensitive chemiluminescence (CL) method for evaluation of medical radiation damage degree is presented. According to the principle of cell stress response to ionizing radiation, lymphocytes will produce reactive oxygen species (ROS) after irradiation. The ROS produced can react with 2‐methyl‐6‐( p ‐methoxyphenyl)‐3,7‐dihydroimidazo[1,2‐ α ] pyrazin‐3‐one (MCLA), a specic CL probe for superoxide anion (O ·‐ 2 ) and singlet oxygen ( 1 O 2 ), to emit light at 465 nm. The CL intensity is positively related to the amount of generated ROS detected 30 min after irradiation. Cell viability, which is inversely related to cell mortality, was determined by MTT assay after 3 days' culture. The results show that both CL intensity and cell mortality of lymphocytes increase with the increase of the radiation dose when the dosage is no more than 3 Gy, suggesting a positive relationship between the degree of lymphocyte cell damage and the amount of ROS generated. In addition, the effects of catalase, Cu–Zn superoxide dismutase (SOD), mannitol, sodium azide (NaN 3 ), and D 2 O on MCLA‐dependent CL of lymphocytes are discussed. We believe that the MCLA‐dependent CL method would potentially provide an easy way for evaluating the degree of lymphocyte damage induced by radiation. Copyright © 2004 John Wiley & Sons, Ltd.