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Flow injection chemiluminescence determination of dihydralazine sulphate based on permanganate oxidation sensitized by rhodamine B
Author(s) -
Yang XiaoFeng,
Wu DongBing,
Li Hua
Publication year - 2004
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.781
Subject(s) - chemiluminescence , permanganate , chemistry , potassium permanganate , detection limit , rhodamine b , chromatography , rhodamine , inorganic chemistry , catalysis , organic chemistry , fluorescence , physics , photocatalysis , quantum mechanics
A novel ow injection chemiluminescence (CL) method for the determination of dihydralazine sulphate (DHZS) is described. The method is based on the CL produced during the oxidation of DHZS by acidic permanganate solution in the presence of rhodamine B. Rhodamine B is suggested as a uorescing compound for the energy‐transferred excitation. The CL emission allows quantitation of DHZS concentration in the range 5–800 ng/mL, with a detection limit of 1.9 ng/mL (3 σ ). The experimental conditions for the CL reaction are optimized and the possible reaction mechanism is discussed. The method has been applied to the determination of DHZS in pharmaceutical preparations and compares well with the high performance liquid chromatography (HPLC) method. Copyright © 2004 John Wiley & Sons, Ltd.