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Scavenger effect of flavonols on HOCl‐induced luminol chemiluminescence
Author(s) -
Selloum Laïd,
Djelili Hanifa,
Sebihi Laïla,
Arnhold Juergen
Publication year - 2004
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.772
Subject(s) - chemistry , rutin , kaempferol , luminol , quercetin , flavonols , chemiluminescence , myricetin , scavenger , biochemistry , antioxidant , nuclear chemistry , chromatography
Abstract Hypochlorous acid (HOCl), the main product of the myeloperoxidase system, is a strong oxidant and a potent chlorinating agent, which can damage host tissues. In the present work, the scavenger effect of three aglycone avonols (myricetin, quercetin and kaempferol) and of the natural glycoside avonol, rutin, was studied towards HOCl using luminol‐dependent chemiluminescence (CL). At 1 µmol/L nal concentration, rutin was the most powerful scavenger of HOCl with an inhibitory luminol oxidation of 91.4% ± 3.2%. Quercetin, kaempferol and myricetin inhibited the luminol‐dependent CL at the same concentration only by 75.9% ± 3.4%, 57.7% ± 5.3% and 43.3% ± 3.5%, respectively. With increasing concentration of these avonols, a dose‐dependent inhibition of luminol CL was observed. In order to prove to what extent avonols scavenge HOCl, their concentrations that gave 50% inhibition of luminescence (IC 50 ) were compared to IC 50 values of the sulphur‐containing compounds N ‐acetyl cysteine (NAC) and taurine. The scavenging activities of compounds tested decrease in the order: rutin > NAC > quercetin > kaempferol > taurine. The present study revealed that rutin was the most effective scavenger agent. Copyright © 2004 John Wiley & Sons, Ltd.