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Discarding multidrug resistance inducers, the possible role of a biosensing reporter in antimicrobial discovery
Author(s) -
Galluzzi Lorenzo,
Virtanen Pekka,
Karp Matti
Publication year - 2004
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.771
Subject(s) - disinfectant , inducer , multiple drug resistance , antimicrobial , plasmid , staphylococcus aureus , drug resistance , biosensor , chemistry , microbiology and biotechnology , antimicrobial drug , combinatorial chemistry , biology , bacteria , biochemistry , gene , organic chemistry , genetics
The aim of the present work was to determine whether the luminescence‐based reporter plasmid pQacLux could be applied to drug discovery in order to discard compounds with dened properties. Non‐pathogenic Staphylococcus aureus RN4220 cells bearing pQacLux were incubated with different concentrations of a disinfectant of common use in hospitals. The in vivo light emission response of the plasmid to the given stimuli was then quantied and compared to a negative control for the construction of dose–response curves. The selected disinfectant provided a convenient model for the activity of quaternary ammonium compounds. In spite of the use of a raw model solution, the system revealed high levels of sensitivity. According to the results obtained, pQacLux could be conveniently used in the rst steps of drug development in order to discard all possible multidrug resistance inducers. Copyright © 2004 John Wiley & Sons, Ltd.