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Chemiluminescence of lucigenin is dependent on experimental conditions
Author(s) -
Hyrsl Pavel,
Lojek Antonin,
Ciz Milan,
Kubala Lukas
Publication year - 2004
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.755
Subject(s) - lucigenin , chemistry , irradiation , chemiluminescence , nuclear chemistry , photochemistry , chromatography , organic chemistry , physics , nuclear physics , enzyme , superoxide
The aim of the study was to test the effect of experimental conditions such as light radiation and temperature on chemiluminescence (CL) of 10 −2 –10 −5 mol/L lucigenin dissolved in various types of solvents. Irradiation by UV light (280, 297, 313 or 400 nm) induced a signicant increase in CL of lucigenin dissolved in borate buffer. This effect was the most obvious for 10 −2 –10 −3 mol/L lucigenin. All wavelengths used had a similar effect. UV irradiation did not induce changes in the CL activity of lucigenin dissolved in dH 2 O or in dimethyl sulphoxide (DMSO). Different results for various solvents were not dependent on pH. The CL activity of 10 −2 mol/L and 10 −3 mol/L lucigenin dissolved in borate buffer increased depending on the solution temperature (25°C, 30°C, 37°C or 40°C) already at the beginning of the analysis, with a further increase during 16 h incubation period. It can be summarized that temperatures higher than 25°C and intensive light irradiation are among those factors which signicantly affect the result of analysis when lucigenin is used as a luminophor. Copyright © 2004 John Wiley & Sons, Ltd.