Premium
Detection of E. coli in beach water within 1 hour using immunomagnetic separation and ATP bioluminescence
Author(s) -
Lee JiYoung,
Deininger Rolf A.
Publication year - 2004
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.753
Subject(s) - bioluminescence , immunomagnetic separation , environmental science , chemistry , contamination , environmental chemistry , chromatography , biology , ecology , biochemistry
The contamination of beach waters occurs from the discharge of storm water and sanitary sewer overows containing faecal material. Additional faecal material derives from discharge of animals and waterfowl. In order to protect public from exposure to faecal‐contaminated water, it is required to test enteric indicators in beach water. The problem is that the traditional culture‐based methods cannot meet this goal because it takes too long (>24 h), so the results are not available until a day later. A rapid method for testing beach water for Escherichia coli within 1 h has been developed. Immunomagnetic separation (IMS) and ATP bioluminescence were used for selective capture and quantication, respectively. This rapid method was compared to the current method (m‐TEC) using beach water samples. The beach samples were preltered with a 20 µm pore size lter in order to remove algae, plant debris and large particles. The results showed that the preltration step did not trap the bacteria which were present in the original water samples. The preltered water was then passed through a 0.45 µm pore size lter for concentration. The deposited bacteria were resuspended and then mixed with superparamagnetic polystyrene beads (diameter of 0.6 µm) that were coated with E. coli antibodies. After IMS, the quantication of the E. coli was done by ATP bioluminescence. The results obtained with IMS‐ATP bioluminescence correlated well with the plate count method (Rsq = 0.93). The detection limit of the assay was about 20 CFU/100 mL, which is well below the US EPA limits for recreational water. The entire procedure can be completed in less than 1 hour. The necessary equipment is portable and was tested on‐site. Copyright © 2004 John Wiley & Sons, Ltd.