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Determination of sulphite using an immobilized enzyme with flow injection chemiluminescence detection
Author(s) -
Yaqoob M.,
Nabi A.,
Waseem A.,
MasoomYasinzai M.
Publication year - 2004
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.752
Subject(s) - chemiluminescence , luminol , detection limit , chemistry , hydrogen peroxide , chromatography , relative standard deviation , flow injection analysis , linear range , calibration curve , analytical chemistry (journal) , organic chemistry
A ow injection method is reported for the determination of sulphite‐based on chemiluminescent detection. Hydro‐gen peroxide is produced from sulphite using on‐line covalently bound immobilized sulphite oxidase packed in a mini‐column, which was mixed downstream and detected via cobalt(II)‐catalysed chemiluminescent oxidation of luminol. The limit of detection (2 × standard deviation of the blank) was 1 × 10 −3 mmol/L with sample throughput 60 h −1 . The calibration data was linear over the range of 0.2–1.0 mmol/L with relative standard deviation ( n = 4) in the range 0.9–2.0%. Copyright © 2004 John Wiley & Sons, Ltd.