Determination of sulphite using an immobilized enzyme with ﬂow injection chemiluminescence detection | Zendy

Yaqoob M. | Zendy; Nabi A. | Zendy; Waseem A. | Zendy; MasoomYasinzai M. | Zendy

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Determination of sulphite using an immobilized enzyme with ﬂow injection chemiluminescence detection

Author(s) -

Yaqoob M.,

Nabi A.,

Waseem A.,

MasoomYasinzai M.

Publication year - 2004

Publication title -

luminescence

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.428

H-Index - 45

eISSN - 1522-7243

pISSN - 1522-7235

DOI - 10.1002/bio.752

Subject(s) - chemiluminescence , luminol , detection limit , chemistry , hydrogen peroxide , chromatography , relative standard deviation , flow injection analysis , linear range , calibration curve , analytical chemistry (journal) , organic chemistry

A ow injection method is reported for the determination of sulphite‐based on chemiluminescent detection. Hydro‐gen peroxide is produced from sulphite using on‐line covalently bound immobilized sulphite oxidase packed in a mini‐column, which was mixed downstream and detected via cobalt(II)‐catalysed chemiluminescent oxidation of luminol. The limit of detection (2 × standard deviation of the blank) was 1 × 10 −3 mmol/L with sample throughput 60 h −1 . The calibration data was linear over the range of 0.2–1.0 mmol/L with relative standard deviation ( n = 4) in the range 0.9–2.0%. Copyright © 2004 John Wiley & Sons, Ltd.

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