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A novel chemiluminescent method for determination of phloroglucinol
Author(s) -
Cui Hua,
Xie Chenggen,
Lai Chunze
Publication year - 2003
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.740
Subject(s) - phloroglucinol , chemiluminescence , luminol , chemistry , detection limit , relative standard deviation , chromatography , organic chemistry
It was found that the inhibition and enhancement by phloroglucinol of the chemiluminescence from the luminol–K 3 Fe(CN) 6 system were dependent on the pH of luminol solution and the concentration of phloroglucinol. In Na 2 CO 3 –NaHCO 3 buffer, phloroglucinol exhibited strong chemiluminescent enhancement at pH 9.4. On this basis, a flow injection method was developed for the determination of phloroglucinol. The method was simple, rapid, convenient and sensitive, with a detection limit of 2.0 × 10 −9 mol/L. It is effective for determining phloroglucinol in the range of 1.0 × 10 −5 –5.0 × 10 −9 mol/L. The relative standard deviation is 1.3% within one day and 3.2% between days for the determination of 5.0 × 10 −7 mol/L phloroglucinol. The method has been successfully used to determine phloroglucinol in environmental water, with satisfactory results. Copyright © 2003 John Wiley & Sons, Ltd.