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The acute response of neutrophil function to a bout of judo training
Author(s) -
Chinda Daisuke,
Umeda Takashi,
Shimoyama Tadashi,
Kojima Arata,
Tanabe Masaru,
Nakaji Shigeyuki,
Sugawara Kazuo
Publication year - 2003
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.739
Subject(s) - cd16 , respiratory burst , zymosan , integrin alpha m , athletes , phagocytosis , opsonin , reactive oxygen species , flow cytometry , immunology , medicine , neutrophile , endocrinology , chemistry , physical therapy , biochemistry , inflammation , cd3 , immune system , in vitro , cd8
Intensive exercise training decreases neutrophil functions in athletes. However, no studies to date have investigated the effect of irregular‐interval training, such as is associated with judo training programmes, on neutrophil functions. The purpose of this study was to examine such effects. Thirty‐seven male college judoists participated in this study. Neutrophil oxidative burst activity, phagocytic activity and expression of CD11b and CD16 per cell were measured by ow cytometry before and after judo training. Total neutrophil counts increased signicantly from 2.98 ± 0.82 to 7.95 ± 1.80 × 10 3 /µL ( p < 0.001). The proportion of neutrophils producing reactive oxygen species (ROS) was increased signicantly ( p < 0.001). On the other hand, the phagocytic activity decreased after training, as shown by a decrease in the amount of ingested opsonized zymosan per cell ( p < 0.001), possibly as a compensatory effect for the increased numbers of ROS‐producing neutrophils. Expression of CD11b and CD16 per cell decreased by 20% and 30%, respectively, after judo training. In conclusion, judo training induced a decrease in phagocytic activity through the lowered expression of CD11b and CD16 on the surface of neutrophils, and increased the oxidative burst activity of neutrophils. Copyright © 2003 John Wiley & Sons, Ltd.