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In vivo monitoring of chlorophyll fluorescence response to low‐dose γ ‐irradiation in pumpkin ( cucurbita pepo ) leaves
Author(s) -
Jovanić B. R.,
Dramićanin M. D.
Publication year - 2003
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.738
Subject(s) - cucurbita pepo , chlorophyll , irradiation , ionizing radiation , chlorophyll a , chemistry , horticulture , chlorophyll fluorescence , cucurbita maxima , botany , biology , physics , biochemistry , nuclear physics
The effect of γ ‐irradiation on the in vivo ‐measured chlorophyll uorescence in a pumpkin leaves ( Cucurbita pepo ) has been investigated. Plants were grown in the same environment, then divided into several groups and irradiated at ambient conditions at small dose levels (up to 13.4 Gy) with 60 Co γ ‐rays. The post‐irradiation effect on chlorophyll status in the leaves was examined by measuring chlorophyll uorescence 2 days (48 h) after exposure. It is undoubtedly found that the value of uorescence intensity ratio (FIR) at 690 nm and 735 nm ( F 690 / F 735 ) depends upon the ionizing radiation dose. Even with the smallest dose of 3.35 Gy, ionizing radiation notably altered the uorescence spectra of leaves. The spectra difference was manifested by decrease of FIR due to changed chlorophyll luminescence, the possible reason for which could be increase of chlorophyll concentration during the recovery process of the plant. The potential implications of these results for plant physiological status monitoring, as well as for pollution detection and assessment, are discussed in brief. Copyright © 2003 John Wiley & Sons, Ltd.