Premium
Expression of firefly luciferase gene in Erwinia amylovora
Author(s) -
Gentilomi Giovanna,
Zeri Lisa,
Ghini Severino,
Zerbini Marialuisa,
Zuffi Elisa,
Musiani Monica,
Girotti Stefano
Publication year - 2003
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.726
Subject(s) - luciferase , erwinia , lac operon , bioluminescence , microbiology and biotechnology , transformation (genetics) , enterobacteriaceae , biology , shuttle vector , infectivity , gene expression , expression vector , bacteria , gene , chemistry , recombinant dna , vector (molecular biology) , escherichia coli , biochemistry , genetics , transfection , virus
In this study we describe an efcient stable genetic transformation of the phytopathogenic bacterium Erwinia amylovora using a recombinant expression vector encoding the rey luciferase gene of Photinus pyralis , which is further controlled by IPTG‐inducible promoter. Stably transformed E. amylovora cells maintain the same infectivity as the wild‐type strain and, after induction with IPTG, produce luciferase. Luminescence produced by the action of luciferase on an exogenous substrate was easily detectable by a simple and rapid bioluminescent assay (BL). The transformed E. amylovora strain maintains the same high emission level, even after passage in pears, until about 15 days post‐infection. Our ndings therefore show that the luciferase assay can be conveniently used to follow the bacterial movement in plant tissue and its dissemination in controlled environments.