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Interaction of aromatic compounds with Photobacterium leiognathi luciferase: fluorescence anisotropy study
Author(s) -
Kudryasheva N. S.,
Nemtseva E. V.,
Visser A. J. W. G.,
van Hoek A.
Publication year - 2003
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.719
Subject(s) - luciferase , chemistry , fluorescence anisotropy , fluorescence , anthracene , photochemistry , molecule , intermolecular force , förster resonance energy transfer , organic chemistry , biochemistry , physics , transfection , quantum mechanics , membrane , gene
Abstract The time‐resolved and steady‐state fluorescence techniques were employed to elucidate possible interactions of four aromatic compounds (anthracene, POPOP, MSB and 1,4‐naphthalendiol) with bacterial luciferase. Fluorescence spectra and fluorescence anisotropy decays of these compounds were studied in ethanol, water–ethanol solutions and in the presence of bacterial luciferase. Shifts of fluorescent spectra and differences in rotational correlation times are interpreted in terms of weak (hydrophobic) interactions of the molecules with the enzyme. These interactions suggest the feasibility of intermolecular energy transfer by an exchange resonance mechanism with a collision‐interaction radius as a way of excitation of these compounds in the reaction catalysed by bacterial luciferase. Copyright © 2003 John Wiley & Sons, Ltd.