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Imaging of hydroperoxide and hydrogen peroxide‐scavenging substances by photon emission
Author(s) -
Yoshiki Yumiko,
Iida Tetsuo,
Akiyama Yoshinobu,
Okubo Kazuyoshi,
Matsumoto Hitoshi,
Sato Michikatsu
Publication year - 2001
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.663
Subject(s) - chemistry , hydrogen peroxide , gallic acid , polyphenol , scavenging , photochemistry , absorption (acoustics) , nuclear chemistry , organic chemistry , antioxidant , physics , acoustics
An imaging system for the hydroperoxide and hydrogen donor was developed by photon emission from the reactive oxygen species–hydrogen donor–KHCO 3 –MeCHO system. Photon intensity (luminance, cd/m 2 ) showed a linear correlation with the concentration of some hydroperoxides [hydrogen peroxide (H 2 O 2 ), tert ‐butyl hydroperoxide and methyl ethyl ketone peroxide] and that of hydrogen donors (catechins and anthocyanins). The linear relationship between photon intensity and concentration was observed in polyphenol‐rich samples, such as teas, berries and currants. Photon intensity from the H 2 O 2 /polyphenol‐rich sample–KHCO 3 –MeCHO system corresponded with H 2 O 2 ‐scavenging activity, rather than with polyphenol contents. It is possible to classify hydrogen donor species according to absorption features using spectral analysis (gallic acid, E max 645 nm, catechins, E max 610 nm; anthocyanins, E max 690–700 nm). This method is a simple and sensitive detection system for hydroperoxide‐ and H 2 O 2 ‐scavenging substances. Copyright © 2001 John Wiley & Sons, Ltd.