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Effect of aqueous cigarette smoke extract on the chemiluminescence kinetics of polymorphonuclear leukocytes and on their glycolytic and phagocytic activity
Author(s) -
Zappacosta Bruno,
Persichilli Silvia,
Minucci Angelo,
Stasio Enrico Di,
Carlino Paola,
Pagliari Gabriella,
Giardina Bruno,
Sole Pasquale De
Publication year - 2001
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.661
Subject(s) - chemiluminescence , chemistry , luminol , zymosan , phagocytosis , aqueous solution , glycolysis , smoke , cigarette smoke , biochemistry , biophysics , extracellular , metabolism , chromatography , microbiology and biotechnology , in vitro , biology , organic chemistry , toxicology
Water‐soluble extracts of cigarette smoke are easily formed in some body compartments, such as saliva or fluid lining alveolar spaces, and can act on both cellular and extracellular compartments. In this paper we have analysed the effect of aqueous smoke extract on some metabolic and functional aspects of polymorphonuclear leukocytes. In particular, the following cellular aspects were studied: chemiluminescence, glycolysis, membrane fluidity and microscopic interaction with zymosan particles. While chemiluminescence and glycolytic activity are highly inhibited, no effect of smoke extract on membrane fluidity was observed. Moreover, the response of luminol‐dependent chemiluminescence was significantly delayed, while that of lucigenin‐dependent chemiluminescence was anticipated. Furthermore, the phagocytic ability of neutrophils pretreated with aqueous smoke extract was also significantly hindered. All these results might indicate that the finely tuned activity of polymorphonuclear leukocytes is somehow hampered by the aqueous extract of cigarette smoke in a way which makes these cells less effective against bacteria and more noxious towards surrounding tissues. Copyright © 2001 John Wiley & Sons, Ltd.