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Effect of interferon‐α on production of reactive oxygen species by human neutrophils
Author(s) -
Koie Takuya,
Suzuki Katsuhiko,
Shimoyama Tadashi,
Umeda Takashi,
Nakaji Shigeyuki,
Sugawara Kazuo
Publication year - 2001
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.606
Subject(s) - reactive oxygen species , interferon γ , production (economics) , chemistry , interferon , oxygen , oxygen metabolism , interferon gamma , virology , biology , biochemistry , in vitro , organic chemistry , economics , macroeconomics
In order to examine that the effects of interferon alpha (IFN‐α) on the production of reactive oxygen species from human neutrophils, we investigated in vitro effects of IFN‐α on neutrophil chemiluminescence responses. Human neutrophils were incubated with 0–1 × 10 5 IU/mL IFN‐α for 10–60 min just before stimulation with opsonized zymosan and phorbol 12‐myristate 13‐acetate. Luminol‐dependent (LmCL) and lucigenin‐dependent (LgCL) chemiluminescence responses were measured using a Lumi Box H‐1000. The LmCL response was inhibited by pre‐incubation with high concentrations of IFN‐α (more than 1 × 10 4 IU/mL). In contrast, LgCL response was increased when neutrophils were pre‐incubated with high concentration of IFN‐α. Effects of pre‐incubation of IFN‐α were similar between CL responses stimulated with OZ and PMA. The increase of LgCL might be due to the inhibitory effect of IFN‐α on myeloperoxidase degranulation, in view of the decreased LmCL responses. Copyright © 2001 John Wiley & Sons, Ltd.