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Enterobacter cloacae leukotoxin: modulation of reactive oxidant species generated by neutrophils
Author(s) -
Barnes Ana Isabel,
Paraje María Gabriela,
Orsilles Miguel,
Albesa Inés
Publication year - 2001
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.604
Subject(s) - enterobacter cloacae , chemistry , stimulation , toxin , reactive oxygen species , chemiluminescence , phorbol , biochemistry , microbiology and biotechnology , chromatography , enterobacteriaceae , enzyme , endocrinology , biology , escherichia coli , protein kinase c , gene
A leukotoxin purified from Enterobacter cloacae culture by saline precipitation, gel chromatography and HPLC was studied as a modulator of reactive oxidant species (ROS) produced by human neutrophils. Chemiluminescence showed that stimulation of ROS was achieved at a low leukotoxin concentration, but ROS production decreased when the toxin was applied at concentrations above 30 µg/mL. Also, the addition of 100 µg toxin/mL significantly reduced the activating effect of phorbol myristate acetate (PMA) and low doses of toxin did not produce an opposite effect toward the stimulation produced by PMA. Normal neutrophils showed a linear correlation between the inverse of ROS production and time, but the kinetic reaction changed when toxins were added to the cells and the ROS formation increased directly with time. Copyright © 2001 John Wiley & Sons, Ltd.