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A signal conversion system using binding‐induced strand displacement for disease biomarker assay
Author(s) -
Jiang Yuling,
Yang Peng,
Du Lijie,
Xia Lingying,
Chen Junbo,
Hou Xiandeng
Publication year - 2021
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.4087
Subject(s) - deoxyribozyme , nucleic acid , dna , signal (programming language) , biomarker , biosensor , streptavidin , chemistry , nanotechnology , computational biology , computer science , biology , materials science , biochemistry , biotin , programming language
Using the principle of binding‐induced DNA strand displacement (BINSD), a DNAzyme‐powered nanomachine biosensor for multiple biomarkers via magnetic beads‐based signal conversion was designed. This sensor can convert multiple biomarker recognition into release of predesigned output nucleic acids tagged with streptavidin proteins (SA‐DNA) for activation of DNA nanomachines. In general, we adopted complementary base pairing rules and affinity ligand specific recognition, and three types of signal conversion systems were constructed that realized universal, sensitive, accurate, and specific detection of multiple biomarkers. Taking the advantage of the strong anti‐interference capability of magnetic separation, this strategy could be used for detection of various biomarkers in clinical practice.