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Benzimidazole‐based turn‐on fluorescence probe developed for highly specific and ultrasensitive detection of hypochlorite ions in living cells
Author(s) -
Yang Yi,
Cheng Siyao,
Dong Wei
Publication year - 2021
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.4063
Subject(s) - fluorescence , fluorophore , chemistry , hypochlorite , benzimidazole , photochemistry , turn (biochemistry) , cytotoxicity , intramolecular force , stereochemistry , inorganic chemistry , biochemistry , organic chemistry , physics , quantum mechanics , in vitro
Hypochlorite (ClO − ), as one of the active oxygen species (ROS), plays an essential role in the cellular defence system and organism immunity. In this paper, we successfully synthesized a new ‘turn‐on’ fluorescent probe BMF based on benzimidazole and characterized it by spectroscopic methods. The designed probe can quickly respond to ClO − with the obvious colour change from pink to colourless. Notably, the probe BMF exhibited almost no fluorescence, but showed strong fluorescence after adding ClO − , including an excellent fluorescence turn‐on effect. The fluorescence turn‐on phenomenon of BMF was attributed to the strong oxidation of ClO − , which severed the connecting double bond and disrupted the intramolecular charge transfer (ICT) system, plus light‐induced electron transfer effect between the fluorophore and the recognition group was discontinued. In addition, the cytotoxicity assay showed that the probe had lower cytotoxicity. Based on these advantages, we demonstrated that probe BMF might be a good candidate for detecting ClO − in biological systems.