Premium
Protein detection based on rolling circle amplification sensors
Author(s) -
Shi Haixia,
Cui Jingjie,
Sulemana Husseini,
Wang Wunian,
Gao Li
Publication year - 2021
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.4017
Subject(s) - rolling circle replication , loop mediated isothermal amplification , template , computer science , nanotechnology , protein detection , signal (programming language) , dna , materials science , dna polymerase , biology , genetics , programming language
Rolling circle amplification (RCA) is an isothermal process under the action of DNA polymerases. Large‐scale DNA templates have been generated using RCA for target detection. Some signal amplification strategies including optical sensors and electrochemical sensors based on RCA have been applied to achieve sensitive detection. Sensors based on RCA have attracted increasing interest. Advances in RCA‐based sensors for protein detection are reviewed in this paper. The advantages and detection mechanisms of sensors based on RCA are revealed and discussed. Finally, possible challenges and future perspectives are also outlined.